KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0173 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
0.0255 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.029 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.0415 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Synechocystis sp. PCC 6803 | Q55366 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + 6 reduced Fdx2 | Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2 | Synechocystis sp. PCC 6803 | ammonia + 2 H2O + 6 oxidized Fdx2 | - |
? | |
nitrite + 6 reduced Fdx2 | Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2. Fdx2 is a more specific electron donor to NiR than ferredoxin | Chlamydomonas reinhardtii | ammonia + 2 H2O + 6 oxidized Fdx2 | - |
? | |
nitrite + 6 reduced ferredoxin | - |
Chlamydomonas reinhardtii | ammonia + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin | - |
Synechocystis sp. PCC 6803 | ammonia + 2 H2O + 6 oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NiR | - |
Chlamydomonas reinhardtii |
NirA | - |
Synechocystis sp. PCC 6803 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.35 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
14.6 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
480 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
666 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 |
General Information | Comment | Organism |
---|---|---|
physiological function | the abundance of NiR polypeptide is increased at least 15fold in nitrate-grown cells | Chlamydomonas reinhardtii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
573 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Chlamydomonas reinhardtii | |
13900 | - |
nitrite | cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
27700 | - |
nitrite | cosubstrate Fdx2, pH 7.5, temperature not specified in the publication | Synechocystis sp. PCC 6803 |